James Anderson, Jofra Archer and Dom Bess will miss the second Test for England against India in Chennai.
It was confirmed on Thursday that Archer will miss out because of an elbow injury, while Bess has been dropped.
Moeen Ali, Stuart Broad, Ben Foakes, Olly Stone and Chris Woakes come into the 12-man squad.
Fast bowler Mark Wood is being rested until the third Test.
Explaining the decision to drop 23-year-old Bess, whose performances have been inconsistent this year, captain Joe Root said: “I’m sure he’ll come back with questions and I’m sure he will be disappointed, but that’s what you expect from guys that really care and want to be out there all the time, performing for their country.
“He’s got a very good attitude. He’s still very much at the start of his career and he’ll use this as an opportunity to get better.”
Archer, who made his Test debut against Australia in 2019, took three wickets in the first-Test triumph but experienced discomfort in his elbow.
He suffered a low-grade stress fracture in his right elbow last year but the current issue is not related to any previous problem and it is hoped the treatment will settle the condition quickly, allowing the Sussex paceman to return in time for the third Test in Ahmedabad starting on 24 February.
His injury is the second late setback England have suffered on the tour, with Zak Crawley taking a tumble on a marble floor and injuring his wrist before the first Test.